Determination of Quercetin and Kaempferol in Honey by HPLC

Objective To establish a method for the determination of quercetin and kaempferd in five kinds of honeys simultaneously.Method The determination was carried out with Hpersil BDS C_(18)(250 mm×4.6 mm,5 μm) column.The mobile phase consisted of methanol+0.4% phosphonic acid(45+55) and pH was adjusted to 4.2 by triethylamine.The flow rate was 1.0(ml/min).The detective wavelength was 375 nm.Results The standard curves of quercetin and kaempferd are linear within the range of 0.045～2.25 μg,0.052～2.600 μg and the coefficient are(0.999?1,)(0.999?1).The reclamation are 95.9%,97.6%.The content s of both quercetin(18.62～189.26(μg/g)) and kaempferol(5.97～22.54(μg/g)) are lower in the five kinds of honey.The contents of quercetin and kaempferol had close relation with the plant that honey came from.The eucalyptus honey has the highest contents of quercetin(189.26(μg/g)),the mangroves honey had the highest contents of kaempferol(22.54(μg/g).) Conclusion The method is simple and accurate and can be used to determine the contents of quercetin and kaempferol in honey simeltaneously.