Determination of ginsenoside Rg1 and Re in Tongru granules by HPLC

Objective To establish a rapid and efficient method for the simultaneous determination of ginsenoside Rg1 and ginsenoside Re in Tongru granules by HPLC. Methods The HPLC analysis was carried out with Agilent ZORBAX SB-C18 and acetonitrile-0.1% phosphoric acid(15:85, V/V) as mobile phase, the flow rate was 1.0 ml/min, the column temperature was 30 ℃, injection volume was 10 μl, and the detection wavelength was 203 nm. Results The Rg1 solution of ginsenoside showed a good linear relation between 0.136-2.040 μg (r2=0.999 9). The ginsenoside Re solution presented a good linear relationship (r2=0.999 8) between 0.066-0.990 μg, with the average addition recovery of 99.3% and 99.1%, and the relative standard deviation of 0.84% and 0.75%(n=6), respectively. Conclusions The method is highly specific, sensitive, reproducible, simple and efficient which can be used for quality control of Tongru Granules. Key words: Ginsenoside Rg1; Ginsenoside Re; High performance liquid chromatography; Tongru granules; Assay (TCD); Quality control