Microarray Analysis of Rice Gene Expression Specific Induced by Xanthomonas oryzae pv. oyzae

The expression profiles of rice (Oryza sativa) near isogenic line (NIL) Zhongzao 14R (resistance) and Zhongzao 9S (susceptible) in response to Xanthomonas oryzae pv. oyzae (Xoo) were studied using cDNA microarray containing 2200 expression sequence tags (ESTs) and ~(33)P hybridization. Results showed that the expression of 315 genes were u-regulated while 176 genes down-regulated among the valid data of 1336 genes after induced for 3 H with Xoo at 4-leaves stage. Further studies of the 78 induced genes with known functions indicated that the changes of gene expression pattern not only reflected coordinating gene regulation in plant defense but also showed the basic model of plant in response to pathogen attack. Some genes never reported to related to the plant defense response while induced by Xoo were reported here by microarray analysis. Novel genes induced by Xoo were also found by using this high-throughput cDNA microarray method.