Therapeutic Use of Tumor Cell-Derived Extracellular Vesicles

Extracellular vesicles (EVs) are nanosized membrane vesicles, released by many cell types, which are an ideal drug-delivery system for antitumor therapy. Exosomes, microparticles (MP), and apoptotic bodiesapoptotic bodies are the three main types of EVs. MPs are EVs with size ranges between 100 and 1000 nm. Tumor cell-derived EVs (T-EVs) can serve as a safe and efficient vehicle to specific deliver chemotherapeutic drugs or oncolytic adenoviruses for tumor treatment. In this section, we describe how to prepare EVs for therapeutictherapeutic use.