Optimpzed Strategies to Hyperexpress Heterologous Protein in Pichia Pastoris

It is very important to produce heterologous proteins both in theory and in practice,expecially for biopharmaceutical.Pichia pastoris has become one of the best eucaryotic expression systems in recent years.The factors influencing the high-level expression of heterologous protein in P.pastoris and the core strategies to hyperexpress heterologous proteins in P.pastoris were discussed in this paper.