Large Scale Culture of Rotavirus by Vero Cell Microcarrier Technique

Objective To culture rotavirus in a large scale by using Vero cell microcarrier technique.Methods Culture Vero cells with microcarrier in a 5 L bioreactor,to which rotavirus strain P[2]G3 was inoculated at a MOI of 0.05 when the cell density reached 1.5×106 cells/ml.Incubate the mixture at 37℃ for 6 d and take samples for observation of CPE and determination of infectious titer of virus daily.Results The infectious titer of virus increased gradually at early stage of culture and reached a peak value 48 h after culture.The virus titer in culture supernatant and lysate of cells were 5.5 and 3.75 CCID50/ml respectively.Conclusion The titer of rotavirus after large scale culture by Vero cell microcarrier technique increased significantly.