Transglutaminase(TGase)-mediated preparation of regenerated protein fibers from wheat gluten and their properties were studied in this work.The effects of TGase concentration gluten concentration,reaction temperature and time on the mechanical properties of wheat gluten(WG) fibers were discussed.The results showed that the fibers obtained a better mechanical property when the gluten concentration was 30wt% and the wheat gluten in the spinning dope was crosslinking treated with 5U/g gluten of TGase for 1h at 50℃.Besides,the fibers exhibited a better water stability at lower temperatures and pH levels.TGase treatment improved the water stability of the fibers.