Isolation,Screening and Identification of the Bacteria with Tolerance of the High-concentration Cr(VI)

[Objective] The research aimed to screen bacteria with tolerance on high-concentration Cr(VI).[Method] Mud with Cr(Ⅵ) at drain of one electroplate factory in Jiangsu as isolation object,a batch of bacteria with tolerance on Cr(Ⅵ) were screened by selection pressure method.Tolerance capacities of the isolated bacteria on different-concentration Cr(Ⅵ) were measured.Taxonomic status of the 9 bacteria was determined.[Result] 26 bacteria with tolerance on Cr(Ⅵ) were screened from Cr-polluted soil,and 9 bacteria could endure Cr(Ⅵ) whose concentration was equal to or more than 300 mg/L.Physiological and biochemical tests and 16S rDNA sequencing result showed that these 9 bacteria respectively belonged to below 6 genera: Arthrobacter,Bacillus,Ochrobactrum,Microbacterium,Amycolatopsis and Pusillimonas.[Conclusion] These 9 bacteria had potential application values in restoration of the Cr(Ⅵ)-polluted habitat.