Production of MLM-type structured lipids by Lipozyme RM IM catalyzed acidolysis

The acidic hydrolysis of soybean oil with caprylic acid and capric acid was utilized to manufacture MLM–type structured lipids using Lipozyme RM IM as the catalyst. Single factor experiments were applied to study the effects of substrates molar ratio,molar ratio of caprylic acidto capric acid,enzyme load,reaction time,reaction temperature and initial water content,giving the following optimum reaction conditions:substrate mole ratio 3∶1(total fatty acid/soybean oil),molar ratio of caprylic acid to capric acid 2.5∶1,enzyme load 7.5 wt%(of the substrate mass),5 h,65 ℃,and initial water content 1 wt%(of the substrate mass). The production contains caprylic acid 20.0 wt%,capric acid 10.5 wt%,and their mass ratio is 1.92.