Preparation and Physicochemical Properties of Chimeric TNT-3 Monoclonal Antibody-modified Sterically Stabilized Liposomes

Chimeric TNT-3 monoclonal antibodies (chTNT-3) were covalently linked to the surface of doxorubicin- entrapped liposomes to prepare chTNT-3 modified sterically stabilized liposomes. The size distribution of the product was (123.1±28.4)nm and the entrapment efficiency was more than 98%. The prepared liposomes showed rather stable physi- cochemical properties when stored at 4℃ for 7 days. Under this condition, liposome size and size distribution did not noticeably change, leakage of drug from liposomes was less than 3%, and immunoreactivity was maintained.