活性氧
过氧化物酶
过氧化氢酶
耐旱性
生物化学
转录因子
生物
发起人
超氧化物歧化酶
细胞生物学
基因
化学
基因表达
植物
酶
作者
Zheng Cheng,Xuming Wang,Yan Xu,Shaomin Wang,Xin Jiang,Xiuli Liu,Weijun Cui,Yueyan Wu,Chengqi Yan,Hongjia Liu,Lu Yin,Jianping Chen,Jie Zhou
标识
DOI:10.1016/j.plaphy.2023.108138
摘要
Drought is among the most severe environmental stressors, imposing detrimental effects on plant growth and development. In this study, we have identified a class III peroxidase gene, OsPrx114, which was induced under PEG (Polyethylene glycol) and drought conditions and found to be localized in both the plasma membrane and endoplasmic reticulum. The promoter region of OsPrx114 encompasses cis-elements, including ABRE (ABA response elements), MBS (MYB binding elements), and W-box, which are likely contributors to drought tolerance. In comparison to Nipponbare (Nip) plants, the overexpression of OsPrx114 enhanced drought tolerance by reducing the accumulation of reactive oxygen species (ROS) through the upregulation of enzyme activities, such as peroxide dismutase (POD) and catalase (CAT). This was accompanied by an increase in proline (Pro) content and a decrease in malondialdehyde (MDA) content. Furthermore, a series of assays including yeast one-hybrid, electrophoretic mobility shift, and dual luciferase assays, demonstrated that the transcription factor OsWRKY50 binds to the W-box (TTGACC) within the promoter of OsPrx114, thereby activating its transcription. OsWRKY50 plays a positive role in regulating OsPrx114-mediated drought resistance by mitigating ROS accumulation in rice. These findings offer a molecular foundation for comprehending the function of the OsWRKY50-OsPrx114 module in response to drought stress in rice.
科研通智能强力驱动
Strongly Powered by AbleSci AI