小桶
AKT1型
PI3K/AKT/mTOR通路
MAPK/ERK通路
药理学
细胞凋亡
蛋白激酶B
根(腹足类)
信号转导
化学
对接(动物)
体外
计算生物学
医学
生物
基因
生物化学
基因表达
基因本体论
植物
护理部
作者
Yang Yang,Mengrong Xu,Wenting Yuan,Feng Yue,Yanglong Hou,Fang Fan,Shili Duan,Lu Bai
出处
期刊:Combinatorial Chemistry & High Throughput Screening
[Bentham Science]
日期:2023-10-19
卷期号:26
标识
DOI:10.2174/0113862073258863230921180641
摘要
Multiple brain disorders are treated by Scutellaria Radix (SR), including cerebral ischemia-reperfusion (CI/R). However, more studies are needed to clarify the molecular mechanism of SR for CI/R.The active substances and potential targets of SR and CI/R-related genes were obtained through public databases. Overlapping targets of SR and CI/R were analyzed using proteinprotein interaction (PPI) networks. GO and KEGG enrichment analyses were performed to predict the pathways of SR against CI/R, and the key components and targets were screened for molecular docking. The results of network pharmacology analysis were verified using in vitro experiments.15 components and 64 overlapping targets related to SR and CI/R were obtained. The top targets were AKT1, IL-6, CAS3, TNF, and TP53. These targets have been studied by GO and KEGG to be connected to a number of signaling pathways, including MAPK, PI3K-Akt pathway, and apoptosis. Molecular docking and cell experiments helped to further substantiate the network pharmacology results.The active compound of SR was able to significantly decrease the apoptosis of HT22 cells induced by OGD/R. This finding suggests that SR is a potentially effective treatment for CI/R by modulating the MAPK and PI3K-Akt pathways.
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