The aim of this study was to investigate the effects of the addition of guar gum (GG) and xanthan gum (XG) on the proximate composition, texture, viscosity, syneresis, color characteristics, microbiological stability, sensory evaluation, rheological properties, and microstructure of a low-fat yogurt sample. The results showed that adding GG and XG at concentrations of 0.5 and 1% increased the hardness and viscosity of yogurt significantly (p < 0.05), with XG having a more pronounced effect. There was no significant difference (p > 0.05) in color characteristics between all treatments during the storage period. The viability of probiotics was enhanced in gum-supplemented yogurts, with XG providing better protection for Lactobacillus delbrueckii subsp. bulgaricus and Bifidobacterium bifidum during storage. Sensory evaluation results showed that XG samples gained higher scores as compared to GG samples. Rheological analysis revealed that both the hydrocolloids guar gum (GG) and xanthan gum (XG) significantly increased the parameters such as viscosity and yield stress of low-fat yogurt, with xanthan gum having a more pronounced effect on enhancing the flow behavior. Microstructural analysis using scanning electron microscopy revealed that XG supplementation improved the yogurt gel network, developing a more compact and cohesive structure; however, GG produced a looser and more dispersed network. Overall, both GG and XG enhanced the rheological, textural, and microstructural characteristics of low-fat yogurt, with XG showing superior effects on texture, gel structure, and probiotic stability.