类有机物
髓母细胞瘤
脑瘤
医学
生命银行
精密医学
个性化医疗
癌症研究
癌症
病理
生物信息学
生物
内科学
神经科学
作者
Chiara Lago,Aniello Federico,Gianpiero Di Leva,Norman Mack,Benjamin Schwalm,Claudio Ballabio,Matteo Gianesello,Luana Abballe,Isabella Giovannoni,Sofia Reddel,Sabrina Rossi,Nicholas T. Leone,Andrea Carai,Angela Mastronuzzi,Alessandra Bisio,Alessia Soldano,Concetta Quintarelli,Franco Locatelli,Marcel Kool,Evelina Miele,Luca Tiberi
标识
DOI:10.15252/emmm.202318199
摘要
Abstract Brain tumors are the leading cause of cancer‐related death in children. Experimental in vitro models that faithfully capture the hallmarks and tumor heterogeneity of pediatric brain cancers are limited and hard to establish. We present a protocol that enables efficient generation, expansion, and biobanking of pediatric brain cancer organoids. Utilizing our protocol, we have established patient‐derived organoids (PDOs) from ependymomas, medulloblastomas, low‐grade glial tumors, and patient‐derived xenograft organoids (PDXOs) from medulloblastoma xenografts. PDOs and PDXOs recapitulate histological features, DNA methylation profiles, and intratumor heterogeneity of the tumors from which they were derived. We also showed that PDOs can be xenografted. Most interestingly, when subjected to the same routinely applied therapeutic regimens, PDOs respond similarly to the patients. Taken together, our study highlights the potential of PDOs and PDXOs for research and translational applications for personalized medicine.
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