Preparation of TEMPO-partially oxidized sodium alginate/SF scaffolds with degradation compatibility for skin defect repair

This study aimed to curtail the secondary damage caused by traditional dressings by cross-linking TEMPO-mediated oxidized sodium alginate (TOSA) with silk fibroin (SF) through the EDC system and preparing the scaffolds through freeze-drying and anhydrous ethanol immersion. The good adsorption, mechanical properties, high porosity, and good in vitro and in vivo biocompatibility of the scaffold can improve the healing efficiency of the wound. Additionally, sodium alginate was oxidized by the TEMPO system to lower its molecular weight and hence increase the degradation rate of the scaffold. Consequently, the scaffold demonstrated a rapid degradation rate (42.26 % degradation in 7 days) in the in vitro enzyme solution. The scaffold will not induce secondary damage to the wound and does not require removal. Furthermore, the oxidation degree of sodium alginate was regulated by the TEMPO system, supporting the preparation of degradation-adapted skin tissue engineering scaffolds.