等温滴定量热法
化学
无规线圈
圆二色性
差示扫描量热法
离子强度
离解(化学)
天蚕素
量热法
位阻效应
结晶学
肽
抗菌肽
立体化学
有机化学
水溶液
物理化学
热力学
生物化学
物理
作者
Adam Jugl,Miloslav Pekař
标识
DOI:10.1016/j.ijbiomac.2022.12.144
摘要
Interactions between hyaluronan and the antimicrobial peptide cecropin B were studied in water and PBS using high-resolution ultrasonic spectroscopy and isothermal titration calorimetry. Although each technique is fundamentally different, they both gave identical results. It was found that the molecular weight of hyaluronan plays an important role in the interactions - in particular, the transition between the rod conformation and the random coil conformation. In water, interactions were saturated in a molar charge ratio of 1.5 and not 1.0 as expected. The later saturation of the interaction probably occurred either for steric reasons or due to the interaction between functional groups in the cecropin structure, which allowed complete dissociation of the antimicrobial peptide. In PBS, in contrast to water, no interactions were observed, irrespective of the molecular weight of hyaluronan. Thus, at a sufficiently high ionic strength, the interactions were suppressed.
科研通智能强力驱动
Strongly Powered by AbleSci AI