检出限
病毒学
抗原
化学
注意事项
适体
严重急性呼吸综合征冠状病毒2型(SARS-CoV-2)
检测点注意事项
冠状病毒
病毒
2019年冠状病毒病(COVID-19)
分子生物学
传染病(医学专业)
色谱法
生物
疾病
免疫学
医学
病理
护理部
作者
Pengcheng Guan,Hong Zhang,Zhiyong Li,Shan-Shan Xu,Miao Sun,Xian-Min Tian,Zhan Ma,Jintong Lin,Man-Man Gu,Wen Huang,Fan‐Li Zhang,Yue-Jiao Zhang,Guangjun Yu,Chaoyong Yang,Zhanxiang Wang,Yanling Song,Jian‐Feng Li
出处
期刊:Analytical Chemistry
[American Chemical Society]
日期:2022-12-13
卷期号:94 (51): 17795-17802
被引量:11
标识
DOI:10.1021/acs.analchem.2c03437
摘要
Addressing the spread of coronavirus disease 2019 (COVID-19) has highlighted the need for rapid, accurate, and low-cost diagnostic methods that detect specific antigens for SARS-CoV-2 infection. Tests for COVID-19 are based on reverse transcription PCR (RT-PCR), which requires laboratory services and is time-consuming. Here, by targeting the SARS-CoV-2 spike protein, we present a point-of-care SERS detection platform that specifically detects SARS-CoV-2 antigen in one step by captureing substrates and detection probes based on aptamer-specific recognition. Using the pseudovirus, without any pretreatment, the SARS-CoV-2 virus and its variants were detected by a handheld Raman spectrometer within 5 min. The limit of detection (LoD) for the pseudovirus was 124 TU μL–1 (18 fM spike protein), with a linear range of 250–10,000 TU μL–1. Moreover, this assay can specifically recognize the SARS-CoV-2 antigen without cross reacting with specific antigens of other coronaviruses or influenza A. Therefore, the platform has great potential for application in rapid point-of-care diagnostic assays for SARS-CoV-2.
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