概念
CYP17A1型
生物
芳香化酶
胎盘
男科
基因亚型
甲酰胺类
雌激素
胎儿
内分泌学
怀孕
基因
遗传学
医学
甲酰胺
癌症
乳腺癌
作者
Caroline Gomes Lucas,Riley M Sullivan,Caroline A Pfeiffer,Paula Chen,JaeWoo Kim,Mariana Sponchiado,Alan J Conley,Randall S. Prather,Kevin D. Wells,Rodney D. Geisert
出处
期刊:Reproduction
[Bioscientifica]
日期:2023-07-27
卷期号:166 (4): 263-269
摘要
In brief Aromatase catalyzes the synthesis of estrogens and has been shown to have an important role during the establishment of pregnancy in the pig. This study confirmed the differential expression of the three aromatase isoforms. Abstract Although three porcine aromatase isoforms have been identified, their gene expression profiles in reproduction are still poorly understood. Here, we identified by Sanger sequencing unique nucleotide signatures for the three paralogous copies of Cyp19 and analyzed by RT-PCR the occurrence of the Cyp19 and Cyp17a1 transcripts at different tissues and stages of conceptus and fetal–placental development. Cyp19a1 and Cyp19a3 expressions were detected in conceptuses and gonads, respectively. Cyp19a2 transcripts were identified on both the conceptuses and the placenta samples. Transcripts for Cyp17a1 were detected predominantly in conceptus and gonads. In the endometrium of day 21 pregnant females, as well as days 12 and 17 pseudopregnant females, we did not detect the expression of Cyp19a1 , Cyp19a2, or Cyp19a3. In our study, we have demonstrated distinct transcriptional regulation for the three functional Cyp19 paralogs and a potential role for Cyp17a1 in controlling the secretion of estrogen from the conceptus and the placenta.
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