荧光
费斯特共振能量转移
斯托克斯位移
光化学
接受者
罗丹明
化学
共振(粒子物理)
衍生工具(金融)
荧光寿命成像显微镜
罗丹明6G
罗丹明B
化学物理
原子物理学
光学
物理
经济
催化作用
凝聚态物理
金融经济学
光催化
生物化学
作者
Xiuji Wang,Lijian Zhang,Qin Li,Yongping Gao
标识
DOI:10.1016/j.dyepig.2020.108614
摘要
Herein, we design and synthesize a pH-dependent turn-on fluorescent probe based on the dark resonance energy transfer (DRET) strategy. A silacyclopentadiene (silole) derivative with aggregation-induced emission (AIE) properties is selected as dark energy donor and a rhodamine B derivative with spirocyclic structure as energy acceptor. Attributed to the non-emissive character of silole in solution, fluorescence leakage from the donor is eliminated. Binding with H+ turns on the DRET process and energy of donor is converted to the acceptor's fluorescence output before non-radiative decay. The probe responds to pH variation from 2.75 to 5 in buffer solutions with a Stokes shift up to 220 nm. In addition, imaging experiments suggest that the probe is capable of determining pH fluctuations in live cells with good cell membrane permeability and low cytotoxicity.
科研通智能强力驱动
Strongly Powered by AbleSci AI