木犀草素
过氧化物酶
化学
对接(动物)
类黄酮
IC50型
生物化学
猝灭(荧光)
立体化学
酶
荧光
体外
抗氧化剂
护理部
物理
医学
量子力学
作者
Fengmao Li,Yu‐Fan Fu,Hao Yang,Tang Yun-ming
标识
DOI:10.1016/j.ijbiomac.2020.10.262
摘要
Luteolin, a plant-derived flavonoid, was found to exert effective inhibitory effect to peroxidase activity in a non-competitive manner with an IC50 of (6.62 ± 0.45) × 10-5 mol L-1. The interaction between luteolin and peroxidase induced the formation of a static complex with a binding constant (Ksv) of 7.31 × 103 L mol-1 s-1 driven by hydrogen bond and hydrophobic interaction. Further, the molecular interaction between luteolin and peroxidase resulted in intrinsic fluorescence quenching, structural and conformational alternations which were determined by multispectroscopic techniques combined with computational molecular docking. Molecular docking results revealed that luteolin bound to peroxidase and interacted with relevant amino acid residues in the hydrophobic pocket. These results will provide information for screening additional peroxidase inhibitors and provide evidence of luteolin's potential application in preservation and processing of fruit and vegetables and clinical disease remedy.
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