Modulation of Gut Microbiota by Soybean 7S Globulin Peptide That Involved Lipopolysaccharide–Peptide Interaction

Soybean protein exhibits nutritional significance for the control of metabolic syndrome, and evidence suggests that gut microbiota are implicated in the control of metabolic disorders. This study aimed to investigate the modulation of pepsin-released peptides of soybean 7S globulin on gut microbiota and possible association between changes of gut microbiota composition and lipopolysaccharide (LPS)–peptide interaction. In vitro fermentation experiments showed that the extension region (ER) fragments of soybean 7S globulin selectively suppressed proinflammatory Gram-negative bacteria. ER peptides also promoted the highest production of short-chain fatty acids (SCFAs), which were associated with increase of the relative abundance of Lachnospiraceae and Lactobacillaceae. Isothermal titration calorimetry (ITC) and Langmuir monolayer studies demonstrated that ER peptides exhibited high affinity to LPS in the presence of Ca2+ and developed into β-sheet-rich aggregate structures, thus weakening the stability of LPS monolayers. This finding supplies a possible explanation for improvement of the effects of soybean 7S globulin on metabolic disease.