微生物群
生物
拟杆菌
厚壁菌
人体微生物群
微生物学
专性厌氧菌
肠道菌群
粘液
厌氧菌
肠上皮
无氧运动
细菌
细胞生物学
免疫学
上皮
生物信息学
生态学
生理学
遗传学
16S核糖体RNA
作者
Hossein Baharvand,Francesca S. Gazzaniga,Elizabeth Calamari,Diogo M. Camacho,Cicely Fadel,Amir Bein,Ben Swenor,Bret Nestor,Michael J. Cronce,Alessio Tovaglieri,Oren Levy,Katherine E. Gregory,David T. Breault,Joaquim M. S. Cabral,Dennis L. Kasper,Richard M. Novak,Donald E. Ingber
标识
DOI:10.1038/s41551-019-0397-0
摘要
The diverse bacterial populations that comprise the commensal microbiome of the human intestine play a central role in health and disease. A method that sustains complex microbial communities in direct contact with living human intestinal cells and their overlying mucus layer in vitro would thus enable the investigation of host–microbiome interactions. Here, we show the extended coculture of living human intestinal epithelium with stable communities of aerobic and anaerobic human gut microbiota, using a microfluidic intestine-on-a-chip that permits the control and real-time assessment of physiologically relevant oxygen gradients. When compared to aerobic coculture conditions, the establishment of a transluminal hypoxia gradient in the chip increased intestinal barrier function and sustained a physiologically relevant level of microbial diversity, consisting of over 200 unique operational taxonomic units from 11 different genera and an abundance of obligate anaerobic bacteria, with ratios of Firmicutes and Bacteroidetes similar to those observed in human faeces. The intestine-on-a-chip may serve as a discovery tool for the development of microbiome-related therapeutics, probiotics and nutraceuticals. A microfluidic intestine-on-a-chip that allows the control of physiologically relevant oxygen gradients, enables the extended coculture of living human intestinal epithelium with stable communities of aerobic and anaerobic human gut microbiota.
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