Studies on antivenom activity of Andrographis paniculata and Aristolochia indica plant extracts against Daboia russelli venom by in vivo and in vitro methods

Methanolic extracts of Andrographis paniculata and Aristolochia indica plants were tested for antivenom activity against Daboia russelli venom. Both plant extracts effectively neutralized the D. russelli venom induced lethal activity. About 0.15 mg of A. paniculata and 0.14 mg of A. indica plant extracts were able to completely neutralize the lethal activity of 2LD50 of D. russelli venom. Various pharmacological activities including edema, haemorrhagic, coagulant, fibrinolytic and phospholipase activities were studied and these pharmacological activities were significantly neutralized by both the plant extracts. The above observations confirmed that A. paniculata and A. indica plant extracts possess potent snake venom neutralizing capacity and could potentially be used for therapeutic purposes in case of snakebite envenomation. Keywords: AntiVenom, plant extracts, snake bite