网织红细胞
细胞生物学
胞吐
小泡
内体
生物
糖蛋白
微泡
化学
生物化学
分泌物
膜
细胞内
核糖核酸
小RNA
基因
作者
Rebecca E. Griffiths,Sabine Kupzig,Nicola Cogan,Tosti J. Mankelow,Virginie M S Betin,Kongtana Trakarnsanga,Edwin Massey,Jon D. Lane,Stephen Parsons,David J. Anstee
出处
期刊:Blood
[American Society of Hematology]
日期:2012-06-28
卷期号:119 (26): 6296-6306
被引量:152
标识
DOI:10.1182/blood-2011-09-376475
摘要
The erythrocyte is one of the best characterized human cells. However, studies of the process whereby human reticulocytes mature to erythrocytes have been hampered by the difficulty of obtaining sufficient numbers of cells for analysis. In the present study, we describe an in vitro culture system producing milliliter quantities of functional mature human adult reticulocytes from peripheral blood CD34+ cells. We show that the final stage of reticulocyte maturation occurs by a previously undescribed mechanism in which large glycophorin A–containing vesicles forming at the cytosolic face of the plasma membrane are internalized and fuse with autophagosomes before expulsion of the autophagosomal contents by exocytosis. Early reticulocyte maturation is characterized by the selective elimination of unwanted plasma membrane proteins (CD71, CD98, and β1 integrin) through the endosome-exosome pathway. In contrast, late maturation is characterized by the generation of large glycophorin A–decorated vesicles of autophagic origin.
科研通智能强力驱动
Strongly Powered by AbleSci AI