化学
检出限
细胞外小泡
线性范围
CD81号
乳腺癌
色谱法
癌症
免疫学
内科学
细胞生物学
医学
生物
病毒
丙型肝炎病毒
作者
Francisco G. Ortega,Matías Regiart,A. Rodríguez–Martínez,Diego Pérez,María José Serrano,José A. Lorente,Gonzalo Tortella,Olga Rubilar,Karim Sapag,Mauro Bertotti,Martín A. Fernández‐Baldo
标识
DOI:10.1021/acs.analchem.0c04180
摘要
This study is focused on identifying novel epithelial markers in circulating extracellular vesicles (EVs) through the development of a dual sandwich-type electrochemical paper-based immunosensor for Claudin 7 and CD81 determination, as well as its validation in breast cancer (BC) patients. This immunosensor allows for rapid, sensitive, and label-free detection of these two relevant BC biomarkers. Under optimum conditions, the limit of detection for Claudin 7 was 0.4 pg mL–1, with a wide linear range of 2 to 1000 pg mL–1, while for CD81, the limit of detection was 3 pg mL–1, with a wide linear range of 0.01 to 10 ng mL–1. Finally, we validated Claudin 7 and CD81 determination in EVs from 60 BC patients and 20 healthy volunteers, reporting higher diagnostic accuracy than the one observed with classical diagnostic markers. This analysis provides a low-cost, specific, versatile, and user-friendly strategy as a robust and reliable tool for early BC diagnosis.
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