胶体金
核化学
材料科学
表面等离子共振
DPPH
纳米医学
MTT法
Zeta电位
傅里叶变换红外光谱
体外
纳米颗粒
纳米技术
化学
生物化学
抗氧化剂
化学工程
工程类
作者
Scholastica Onyebuchi Anadozie,Olusola Bolaji Adewale,Mervin Meyer,Hajierah Davids,Saartjie Roux
出处
期刊:Nanotechnology
[IOP Publishing]
日期:2021-05-14
卷期号:32 (31): 315101-315101
被引量:11
标识
DOI:10.1088/1361-6528/abf6ee
摘要
Abstract The development of gold nanoparticles (AuNPs) using a green approach has drawn considerable interest in the field of nanomedicine. Its wide application in clinical diagnosis, imaging and therapeutics portrays its importance for human existence. In this study, we reported on the biogenic synthesis of AuNPs using the aqueous extract of the Xylopia aethiopica fruit (AEXAf), which acts as both a reducing and stabilizing agent. The characterization of AEXAf-AuNPs was performed using ultraviolet-visible spectroscopy, dynamic light scattering and zeta potential measurements, high-resolution transmission electron microscopy and Fourier transform-infrared spectroscopy. The i n vitro anti-oxidant activities of the AEXAf-AuNPs and AEXAf were evaluated using 2,2-diphenyl-1-picrylhydrazyl (DPPH) and ferric reducing anti-oxidant power. The in vitro cytotoxic activities of the AEXAf-AuNPs and AEXAf against breast and colorectal cancer cells were evaluated using 3,-(4,5 dimethylthiazol)-2,5 diphenyl tetrazolium bromide (MTT) viability and annexin V/PI assays. The AEXAf-AuNPs exhibited surface plasmon absorption maximum at 522 nm and were stable for 4 weeks. The average size of the AEXAf-AuNPs was 10.61 ± 3.33 nm on the high-resolution transmission electron microscopy images. The in vitro anti-oxidant activities of the AEXAf-AuNPs and AEXAf were concentration dependent. The AEXAf-AuNPs were cytotoxic to the cancer cells and non-toxic to the non-cancerous human fibroblast cells (KMST-6) (up to 200 μ g ml −1 ). From these results, the AEXAf-AuNPs showed good anti-oxidant and anti-cancer activities, and can be suggested as a possible therapeutic agent for breast and colorectal cancer.
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