山奈酚
乙酸乙酯
抗菌剂
卡西亚
大黄素
生物
疫病疫霉菌
植物
化学
传统医学
类黄酮
微生物学
抗氧化剂
生物化学
枯萎病
医学
替代医学
中医药
病理
作者
Duong Quang Pham,Hieu Pham,Jae Woo Han,Tung H. Nguyen,Huong Thanh Nguyen,Thi Duyen Nguyen,Thu Trang Nguyen,Cuong Tu Ho,Hong Minh Pham,Vũ Đình Hoàng,Gyung Ja Choi,Quang Lê Đăng
标识
DOI:10.1016/j.indcrop.2021.113465
摘要
The use of synthetic chemicals in agriculture has not only caused humans and animals’ health risks but also environmental pollution. Botanical pesticides have been considered as alternatives to harmful pesticides because of their low toxicity and easy biodegradable property. The objective of this study was to evaluate the antimicrobial activities of the extracts and metabolites from the leaves of Cassia alata L. against fungal and bacterial phytopathogens. Ethyl acetate-soluble extract derived from C. alata leaves has been found to remarkably control the plant diseases caused by fungi Magnaporthe oryzae, Phytophthora infestans, Colletotrichum coccodes and Puccinia recondita in vivo. To provide scientific evidence on the effectiveness of the ethyl acetate-soluble extract against plant pathogens, the bioassay-guided fractionation of the extract was conducted and thereby eight compounds including methyl 2,4,6-trihydroxybenzoate (1), aloe-emodin (2), kaempferol (3), (-)-epiafzelechin (4), rhein (5), kaempferol-3-O-glycoside (6), kaempferol-3-O-gentiobiside (7) and aloe-emodin-8-O-β-D-glucoside (8) were identified. The content of 2, 5, and 8 in the ethyl acetate-soluble extract and methanol extract of C. alata leaves were quantified by HPLC analysis. Compounds 3-8 displayed in vitro antifungal activity against M. oryzae and Phytophthora species. Out of the isolated compounds, rhein (5) displayed the best antifungal activity; it strongly inhibited in vitro the mycelial growth of Phytophthora species and effectively suppressed tomato late blight (TLB) by 57.1 % at 150 μg/mL and 87.9 % at 300 μg/mL on the tomato seedlings in vivo. Besides, rhein showed excellent in vitro inhibition for the bacterial growth of Acidovorax avenae subsp. cattlvae with an IC50 of 2.5 μg/mL. From the results, it was confirmed that the ethyl acetate-soluble extract derived from C. alata leaves contains antimicrobial constituents against various plant pathogens and has potent in vivo efficacy for controlling plant diseases.
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