蛋白质聚集
过氧化氢
化学
氧化应激
活性氧
氧化磷酸化
荧光
次氯酸
生物物理学
生物化学
内生
氧化剂
MSRA公司
细胞生物学
生物
氨基酸
物理
有机化学
量子力学
蛋氨酸
作者
Yubo Huang,Jichun Wu,Y Zhang,Wenjing Ding,Binbin Wang,Jun Wan,Yaqiong Yang,Baoxing Shen
标识
DOI:10.1016/j.snb.2023.134458
摘要
The misfolding and aggregation of specific proteins induced by endogenous oxidation are closely related to some incurable diseases including cancers, diabetes and neurodegenerative diseases. Reactive oxygen species (ROS), as a common oxidizing substance in vivo would induce the oxidation of endogenous biomacromolecules like proteins. However, it remains a challenge to detect protein aggregation induced by specific ROS. Herein, we demonstrate that the developed probe (P2) with a dual function that can simultaneously detect hydrogen peroxide (H2O2) levels and protein aggregates formed by oxidative stress. In this probe, the boronic acid pinacol ester group was conjugated to fluorescent molecular rotor 4-hydroxybenzylidene-imidazolinone (HBI) and served as the H2O2 detection group. Experimental results show that the fluorescence of P2 can be activated by H2O2 and exhibited a positive correlation with the degree of protein aggregation. Moreover, combined with AggTag method P2 was successfully applied to dual-channel imaging of protein aggregation that is stressed by H2O2 in cellular. In general, this work not only provides a potential method to study protein aggregation that associates with oxidation in live cells but also can be generally applied to study other biological processes.
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