活性氧
脐静脉
炎症
超氧化物歧化酶
氧化应激
肿瘤坏死因子α
男科
内分泌学
生物
内科学
医学
化学
生物化学
体外
作者
Sara E. Mascone,Katherine I. Kim,William S. Evans,Steven J. Prior,Marc D. Cook,Sushant M. Ranadive
出处
期刊:PLOS ONE
[Public Library of Science]
日期:2023-10-04
卷期号:18 (10): e0292112-e0292112
标识
DOI:10.1371/journal.pone.0292112
摘要
Black individuals and men are predisposed to an earlier onset and higher prevalence of hypertension, compared with White individuals and women, respectively. Therefore, the influence of race and sex on reactive oxygen species (ROS) production and superoxide dismutase (SOD) activity following induced inflammation was evaluated in female and male human umbilical vein endothelial cells (HUVECs) from Black and White individuals. It was hypothesized that HUVECs from Black individuals and male HUVECs would exhibit greater ROS production and impaired SOD activity. Inflammation was induced in HUVEC cell lines (n = 4/group) using tumor necrosis factor-alpha (TNF-α, 50ng/ml). There were no between group differences in ROS production or SOD activity in HUVECs from Black and White individuals, and HUVECs from Black individuals exhibited similar SOD activity at 24hr compared with 4hr of TNF-α treatment (p>0.05). However, HUVECs from White individuals exhibited significantly greater SOD Activity (p<0.05) at 24hr as compared to 4hr in the control condition but not with TNF-α treatment (p>0.05). Female HUVECs exhibited significantly lower ROS production than male HUVECs in the control condition and following TNF-α induced inflammation (p<0.05). Only female HUVECs exhibited significant increases in SOD activity with increased exposure time to TNF-α induced inflammation (p<0.05). HUVECs from White individuals alone exhibit blunted SOD activity when comparing control and TNF-α conditions. Further, compared to female HUVECs, male HUVECs exhibit a pro-inflammatory state.
科研通智能强力驱动
Strongly Powered by AbleSci AI