Metal–Organic Framework‐Based Artificial Organelle Corrects Microenvironment Interference for Accurate Intratumoral Glucose Analysis

Abstract Enzymatic catalysis with high efficiency allows them a great prospect in metabolite monitoring in living cells. However, complex tumor microenvironments, such as acidity, H 2 O 2 , and hypoxia, are bound to disturb catalytic reactions for misleading results. Here, we report a spatially compartmentalized artificial organelle to correct intratumoral glucose analysis, where the zeolitic imidazolate framework‐8 immobilized glucose oxidase‐horseradish peroxidase cascade core and catalase‐directed shell act as signal transduction and guarding rooms respectively. The acid‐digested core and stable shell provide appropriate spaces to boost biocatalytic efficiency with good tolerability. Notably, the endogenous H 2 O 2 is in situ decomposed to O 2 by catalase, which not only overcomes the interference in signal output but also alleviates the hypoxic states to maximize glucose oxidation. The marked protective effect and biocompatibility render artificial organelles to correct the signal transduction for dynamic monitoring glucose in vitro and in vivo, achieving our goal of accurate intratumoral metabolite analysis.