葡萄糖氧化酶
化学
细胞器
辣根过氧化物酶
过氧化氢酶
生物相容性
代谢物
生物化学
蛋白质稳态
催化作用
生物物理学
组合化学
酶
有机化学
生物
作者
Weiqing Xu,Yu Wu,Yuling Xu,Xiaoli Cai,Wenling Gu,Chengzhou Zhu
标识
DOI:10.1002/anie.202308827
摘要
Enzymatic catalysis with high efficiency allows them a great prospect in metabolite monitoring in living cells. However, complex tumor microenvironments, such as acidity, H2 O2 , and hypoxia, are bound to disturb catalytic reactions for misleading results. Here, we report a spatially compartmentalized artificial organelle to correct intratumoral glucose analysis, where the zeolitic imidazolate framework-8 immobilized glucose oxidase-horseradish peroxidase cascade core and catalase-directed shell act as signal transduction and guarding rooms respectively. The acid-digested core and stable shell provide appropriate spaces to boost biocatalytic efficiency with good tolerability. Notably, the endogenous H2 O2 is in situ decomposed to O2 by catalase, which not only overcomes the interference in signal output but also alleviates the hypoxic states to maximize glucose oxidation. The marked protective effect and biocompatibility render artificial organelles to correct the signal transduction for dynamic monitoring glucose in vitro and in vivo, achieving our goal of accurate intratumoral metabolite analysis.
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