微泡
伤口愈合
巨噬细胞
小RNA
医学
癌症研究
化学
免疫学
生物化学
基因
体外
作者
Zibo Xu,Tao Ni,Qian Zhang,Xiaowei Sun,Liping Zhao,Jinde Lin,Weicheng Gao,Min Yi,Lantian Zhang,Liying Tu,Guoping Wu,Wei Yan
标识
DOI:10.1016/j.bbadis.2024.167640
摘要
Diabetes is an extremely costly disease, one-third of which are attributed to the management of diabetic foot disease including chronic, non-healing, diabetic foot ulcers (DFUs). Therefore, much effort is needed to understand the pathogenesis of DFUs and novel therapeutics. We utilized exosome staining to confirm the interaction between fibroblast-derived exosomes and macrophages. Subsequently, we employed public data and qPCR to screen for upregulated miRNAs in fibroblast-derived exosomes in DFUs. The relationship between was validate miR-93-5 and ATG16L1 through data prediction and dual-luciferase reporter assays. A variety of molecular biology experiments were used for subsequent pathway validation. Additionally, we established Atg16l1 MKI and Nlrp3 MKO mice for further validation. We identified that miR-93-5p derived from fibroblasts played an important role in M1 macrophages polarization. Predicted by database, we found that miR-93-5p can bind to ATG16L1 mRNA, thereby influencing macrophage autophagy mediated by ATG16L1 in the clearance of ROS, thus activating the NLRP3 signaling pathway. In vivo, miR-93-5p antagomir treatment accelerated diabetic wound healing and induced M2 macrophage polarization. Fibroblasts and macrophages show cell crosstalk during the development of DFUs by miR-93-5p, and that antagomir treatment may be a promising and technically advantageous alternative to DFUs therapies. • There is no effective treatment for DFU, and its pathological mechanism has notyet been elucidated. • How fibroblasts in DFU regulate macrophage function through exosomes. • The mir-93-5p derived from fibroblasts inhibits the transcription of Atg16L1 within macrophages via exosomes, subsequently suppressing autophagy-mediated clearance of reactive oxygen species (ROS), thereby activating Nlrp3. • Our result provides strong evidence for the clinical treatment of DFU patients.
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