Circ_0111277 impedes cell proliferation, migration, and invasion of trophoblast cell by modulating miR‐411‐5p/GADD45A axis in preeclampsia

Abstract Background : Circular RNAs (circRNAs) are crucial supervisory factors that are applied in varied diseases, comprising preeclampsia (PE). This paper reconnoiters the effect of circ_0111277 in trophoblast cell. Methods : The quantitative real‐time PCR and western blot were executed to quantify the abundance of circ_0111277, microRNA‐411‐5p (miR‐411‐5p) and growth arrest and DNA damage inducible alpha (GADD45A). The Cell Counting Kit‐8 assay, colony formation test, and 5‐Ethynyl‐2’‐deoxyuridine assay were carried out to evaluate the cell proliferation. The cell migration and invasion were measured by transwell assay and wound healing assay. Dual‐luciferase reporter assay distinguished the binding between miR‐411‐5p and circ_0111277 or GADD45A. Results : Circ_0111277 and GADD45A were enlarged, but miR‐411‐5p was hampered in PE. Circ_0111277 privation triggered cell proliferation, migration, and invasion, and the miR‐411‐5p inhibitor overturned the consequence of si‐circ_0111277 in HTR‐8/SVneo cells. Moreover, miR‐411‐5p mimic stimulated HTR‐8/SVneo cells growth by adjusting GADD45A expression. Conclusion : Circ_0111277 invigorated PE advancement via miR‐411‐5p/GADD45A. This article is protected by copyright. All rights reserved