GTP'
纳米囊
微管
微管蛋白
鸟苷三磷酸
鸟苷
生物物理学
化学
纳米片
三磷酸腺苷
细胞生物学
生物化学
生物
纳米技术
材料科学
纳米颗粒
酶
有机化学
作者
Noriyuki Uchida,Ai Kohata,Kou Okuro,Annalisa Cardellini,Chiara Lionello,Eric A. Zizzi,Marco Agostino Deriu,Giovanni M. Pavan,Michio Tomishige,Takaaki Hikima,Takuzo Aida
标识
DOI:10.1038/s41467-022-33156-5
摘要
Abstract Nanocapsules that collapse in response to guanosine triphosphate (GTP) have the potential as drug carriers for efficiently curing diseases caused by cancer and RNA viruses because GTP is present at high levels in such diseased cells and tissues. However, known GTP-responsive carriers also respond to adenosine triphosphate (ATP), which is abundant in normal cells as well. Here, we report the elaborate reconstitution of microtubule into a nanocapsule that selectively responds to GTP. When the tubulin monomer from microtubule is incubated at 37 °C with a mixture of GTP (17 mol%) and nonhydrolysable GTP* (83 mol%), a tubulin nanosheet forms. Upon addition of photoreactive molecular glue to the resulting dispersion, the nanosheet is transformed into a nanocapsule. Cell death results when a doxorubicin-containing nanocapsule, after photochemically crosslinked for properly stabilizing its shell, is taken up into cancer cells that overexpress GTP.
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