Optimization of cordycepin extraction from cordyceps militaris fermentation broth

Cordyceps is one of the components of traditional Chinese medicine. This mushroom becomes worldwide famous due to its significant therapeutic effects on various diseases. Nucleoside like cordycepin, a major bioactive compound from Cordyceps militaris, is commonly known as promising anticancer and antitumor compound. The aim of study is to determine an optimal mixture ratio of solvent-solvent-based extraction method, which is used for extraction of cordycepin, from cultivated Cordyceps militaris DSMZ 23612 fermentation broth. Hence, the optimization process was performed using statistical (RSM) design leaded an optimal cordycepin extraction using solvent mixture including hot water (23%), ethanol (30%), methanol (25%) and ethyl acetate (22%). In addition, the final extracted cordycepin (786.94 mgL-1 of 81% purity) was achieved through extraction parameters as sample: solvent ratio (v/v), extraction temperature, extraction time and frequency at (1:10), 35 °C, 90 min and 1 cycle, respectively. The experimental values obtained were fit with those predicted, thus indicate the suitability of RSM and method for optimization of cordycepin extraction.