活性氧
超氧化物
氧化应激
细胞生物学
线粒体ROS
胞浆
化学
线粒体
生物
生物化学
酶
作者
Chih-Yao Chung,Michael R Duchen
出处
期刊:Methods in molecular biology
日期:2022-01-01
卷期号:: 333-337
标识
DOI:10.1007/978-1-0716-2309-1_24
摘要
AbstractIntracellular reactive oxygen species (ROS) act as an important signaling transductor in cells, regulating almost every aspect of cell biology. Measurements of ROS production thus, offer links between oxidative stress and cell pathophysiology. Here, we describe a simple screening assay in intact adherent cells by fluorescence microplate readers, using dihydroethidium (DHE) and MitoSOX to measure cytosolic superoxide and mitochondrial superoxide production, respectively. This assay enables a quick and reliable assessment of ROS generation in a well-controlled environment.Key wordsReactive oxygen speciesDihydroethidiumMitoSOXMitochondriaPlate reader
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