RNA干扰
RNA沉默
生物
小发夹RNA
计算生物学
基因沉默
反式siRNA
基因
RNA诱导沉默复合物
核糖核酸
遗传学
克隆(编程)
功能基因组学
RNA诱导的转录沉默
基因组
基因组学
计算机科学
程序设计语言
作者
Jinping Zhao,Carlos Garcia Rios,Jun Xu,Ijaz Ahmad,Junqi Song
出处
期刊:Methods in molecular biology
日期:2022-01-01
卷期号:: 283-292
标识
DOI:10.1007/978-1-0716-1875-2_18
摘要
RNA interference (RNAi) is an evolutionarily conserved post-transcriptional gene silencing mechanism that responds to double-stranded RNA (dsRNA) by sequence-specific downregulation of target genes. The dsRNA-mediated RNAi technology has become one of the most widely used and powerful tools for functional genomic studies in diverse organisms. However, its application has been limited due to the technical difficulty of making RNAi constructs caused by the inverted repeat structure that is required for the formation of hairpin RNA. Here, we present a ligation-independent cloning-based dual vector-mediated RNAi system for silencing specific genes in plants. This approach is simple, efficient, and cost-effective and can be readily adapted to other binary vectors for functional analysis of target genes and the development of sustainable disease and pest control strategies in a broad range of plant species.
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