Determination of oleanolic acid in Herba Ecliptae by reversed-phase high performance liquid chromatography.

Objective:To develop a method of RP-HPLC for the quantitative analysis of oleanolic acid in Herba Ecliptae.Methods:Oleanolic acid was separated on Phenomenex C18(4.6 mm×250 mm,5 μm) using a mixture of acetonitrile and 0.1% phosphoric acid solution(90:10) as the mobile phase and detected at 210 nm;The flow rate was 1.0 ml/min and 20 μl.sample solution was injected every time.Results:There was good liner relationship between the peak area and concentration at ranges of 0.569-3.414μg(r=0.999 9) for oleanolic acid;the average recovery rate of oleanolic acid was 98.40%(RSD=2.44%).Conclusion:The established method was rapid and simple with good accuracy and reproducibility for the determination of oleanolic acid,the method was suitable for the quality control of Herba Ecliptae.