Concentration of 5-FU in the portal vein blood after intraperitoneal sustained-releasing chemotherapy in rabbit model

Objective To determine the concentration of 5-FU in the portal vein blood after intraperitoneal sustained-releasing chemotherapy in rabbit model.Methods The portal vein blood was collected at different time points after intravenous chemotherapy,intraperitoneal chemotherapy or intraperitoneal sustained-releasing chemotherapy with 5-FU,respectively.The blood concentration of 5-FU was measured by HPLC.The area under curve(AUC) was calculated based on the concentration-time curve.Results The peak blood concentraton of 5-FU in the portal vein was 0.82 μg/ml and reached two hours after intraperitoneal administration of sustained-releasing agents.The concentraton of 5-FU in the portal vein was stable and could be detected untill day 16 later after treatment.In contrast,the average concentration of 5-FU in the portal vein in animals receiving intraperitoneal injection of 5-FU was 20.67 μg/ml 15 minutes after injection and was indetectable 24 hours later.AUC in each group was:AUCintravenous group=21.57(μg/ml·h);AUCintraperitoneal group=42.85(μg/ml·h);AUCsustained-releasing group=70.08(μg/ml·h),respectively.Conclusions Intraperitoneal sustained-releasing chemotherapy might prolong the effective exposure time of 5-FU in the portal vein and enhance the chemotherapeutic effects to the liver.