Preparation and characterization of monoclonal antibody against Vibrio vulnificus

AIM:To prepare monoclonal antibody(mAb)against Vibrio vulnificus and evaluate its roles in rapid diagnosis for Vibrio vulnificus infection.METHODS:Balb/c mice were immunized by inactived Vibrio vulnificus.Anti-Vibrio vulnificus McAbs were prepared by using hybridoma technique.The ELISA was used to identify Ig subgroup,titers of ascites,relative affinity and the specificity of McAbs.RESULTS:Two cell lines of hybridoma were established and they constantly secreted high quality McAbs steadily.CONCLUSION:The McAbs established could be highly specific for Vibrio vulnificus,which not only lays a solid foundation for development of a rapid method for detection of Vibrio vulnificus infection but also provides the theoretical basis for further investigation of the treatment.