Prophyrin self-assembly was proved based on meso-tetra-methoxyphenyl prophyrin fluorescence recovered quenching caused by Hg2+.The fluorescence of meso-tetra-methoxyphenyl prophyrin acetone solution quenching caused by Hg2+,introduced water cause prophyrin self-assembly via hydrogen-bonding ability,then fluorescence recovered.When added Hg2+ rapid decay of fluorescence intensity was observed immediately,but the fluorescence recovered was slow.Periodical fluorescence quenching and recovering can be observed with continual Hg2+ addition.And this phenomenon was demonstrated via fluorescence spectrum and UV spectrum.