拉曼散射
化学成像
显微镜
拉曼光谱
相干反斯托克斯拉曼光谱
信号(编程语言)
光学
调制(音乐)
激光器
材料科学
分辨率(逻辑)
高光谱成像
化学
光电子学
物理
声学
地质学
遥感
人工智能
程序设计语言
计算机科学
作者
Bi-Chang Chen,Jiha Sung,Su Jun Lim
摘要
We present a new coherent anti-Stokes Raman scattering (CARS) method that can perform background-free microscopy and microspectroscopy at the vibrational fingerprint region. Chirped broad-band pulses from a single Ti:sapphire laser generate CARS signals over 800-1700 cm(-1) with a spectral resolution of 20 cm(-1). Fast modulation of the time delay between the pump and Stokes pulses coupled with lock-in signal detection not only removes the nonresonant background but also produces Raman-like CARS signals. Chemical imaging and microspectroscopy are demonstrated with various samples such as edible oils, lipid membranes, skin tissue, and plant cell walls. Systematic studies of the signal generation mechanism and several fundamental aspects are discussed.
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