[Promoter activity of B-cell maturation antigen gene in mice].

To investigate the promoter activity of BCMA gene 5'-flanking regionfor the study the regulation mechanism of BCMA expression.Luciferase reporter plasmids containing 5'-flanking region of BCMA gene and serial deletions of the fragment were constructed. The luciferase expression was observed after these reporters were transfected into J558L, 293T and Hela cells.The highest transcriptional activation was expressed in pGL3-B157 reporter, followed by pGL3-B607, pGL3-B359, pGL3-B93, and pGL3-B820 in sequence. The highest transcriptional activation was in J558L cell.The 5'-flanking sequence from -820 to +145 bp of BCMA is of promoter activity. Serial deletion analysis of the promoter region of BCMA gene suggests the sequence from -93 to +145 could be a core promoter region.