Extraction of Collagen by Pepsin from the Cattle Skin

The collagen from cattle skin has been extracted by using the pepsin,and the composition of cattle skin was also analyzed.Effects of enzyme addition,temperature and extraction time as well as solid-liquor ratio on the extraction efficiency of collagen were investigated in this paper.The optimal processing conditions were obtained by orthogonal experiments as follows:0.5 mol/L acetic acid as extraction solvent,the added pepsin dosage of 3 000 U/g and the ratio of material to liquor 1∶30,extraction at 30 ℃ for 8 h,the recovery yield of the collagens was 61.7%.The maximum absorption wavelength of the sample is 232 nm by UV scan,which is consistent with the Sigma product.