Application research of Lonicerae japonicae flos and Lonicerae flos in seven preparations

Objective:To Establish a TLC method to differentiate Lonicerae japonicae flos and Lonicerae flos;and examine the proportion of Lonicerae japonicae flos in seven preparations of commonly used Traditional Chinese medicines.Methods:Using a HPLC method for ensuring that the macranthoidin B was the distinctive element of Lonicerae flos;and then a TLC method was designed for distinguishing the Lonicerae japonicae flos and the Lonicerae flos.Meanwhile,a TLC method was carried out for exploring the using mode of Lonicerae japonicae flos in seven commonly used traditional Chinese medicines.Results:Lonicerae japonicae flos are used in the Shuanghuanglian Injection of six manufacturers and Lonicerae flos are used in six oral preparations.Conclusion:The methods are simple,accurate,and can determine the application proportions of Lonicerae japonicae flos in the traditional Chinese medicine.