Feasibility Study of TaqMan-MGB Probes in Detection of Genotyping of GSTP1 Exon 5 SNP

Objective: To evaluate TaqMan-MGB Probes genotyping method feasibility in detecting a well-known SNP, compared with PCR-RFLP. Methods: A high-throughput genotyping method has been developed to detect single-nucleotide polymorphisms (SNP). In 321 specimens, GSTP1 exon 5 SNP was analyzed by TaqMan-MGB probes genotyping and PCR-RFLP method. Results: The results of the two methods were completely identical: 226 (70.4%) of homozygous wild-type (AA), 92 (28.7%), heterozygote (AG) and 3 (0.9%) ,homozygous mutant (GG). Conclusion: Genotyping method of TaqMan-MGB Probes is a rapid method, with high specificity,to highly automatically detect SNP,which can be applied to the large-scale genotyping.