Cellulose in jujube powder was hydrolyzed by HCl,NaOH and cellulase of different concentration at different temperature for different time to determine the optimal hydrolysis condition.The optimal concentration of HCl,NaOH and cellulase was 0.05 mol/L,0.5 mol/L and 0.1×10-6 g/g respectively.The optimal temperature for HCl,NaOH and cellulase was 30,30 and 40 ℃ respectively.The optimal time for HCl,NaOH and cellulase was 20 min.There was no significant difference among three methods for cellulose hydrolysis.