Collagen type II induced with two different methods in dedifferentiated chondrocytes

Objective:To induced the marked phenotype collagen type II of chondrocyte in dedifferentiated immortalized human articular chondrocytes(IHACs P50) with two different methods.Method: Dedifferentiated IHACs P50 were induced with the Nutridoma-sp ascorbate free of serum medium and centrifuge aggregate culture, then type I,II, III collagen immunohistochemical stain and reverse transcriptional polymerase chain reaction of type II collagen and collagen quantity were performed.Result:Collagen type II was successfully induced with Nutridoma-sp ascorbate free of serum medium and centrifuge aggregate culture and the later promoted almost the same type II collagen growth compared with the former.Conclusion: Various inducing methods are able to redifferentiated type II collagen in dedifferentiated IHACs, both Nutridoma-sp ascorbate free of serum medium and centrifuge aggregate culture are able to produce type II collagen in dedifferentiated IHACs