体内
刺激
化学
细胞毒性
水溶液
体外
色谱法
肺表面活性物质
皮肤刺激
MTT法
药理学
有机化学
生物化学
皮肤病科
医学
生物
生物技术
免疫学
作者
Anna Czajkowska-Kośnik,Eliza Wolska,Juliusz Chorążewicz,Małgorzata Sznitowska
标识
DOI:10.3109/03639045.2014.938656
摘要
The in vivo model on rabbit eyes and the in vitro cytotoxicity on fibroblasts were used to compare irritation effect of aqueous and oily (Miglyol 812) solutions of surfactants. Tween 20, Tween 80 and Cremophor EL were tested in different concentrations (0.1, 1 or 5%) and the in vitro test demonstrated that surfactants in oil are less cytotoxic than in aqueous solutions. In the in vivo study, the aqueous solutions of surfactants were characterized as non-irritant while small changes in conjunctiva were observed after application the oily solutions of surfactants and the preparations were classified as slightly irritant, however this effect was similar when Miglyol was applied alone. In conclusion, it is reported that the MTT assay does not correlate well with the Draize scores.
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