化学
过氧化氢
肽
体内
连接器
生物物理学
细胞穿透肽
细胞
氧化应激
荧光
生物化学
费斯特共振能量转移
生物技术
物理
操作系统
生物
量子力学
计算机科学
作者
Roy Weinstain,Elamprakash N. Savariar,Csilla N. Felsen,Roger Y. Tsien
摘要
A hydrogen peroxide (H2O2)-activated cell-penetrating peptide was developed through incorporation of a boronic acid-containing cleavable linker between polycationic cell-penetrating peptide and polyanionic fragments. Fluorescence labeling of the two ends of the molecule enabled monitoring its reaction with H2O2 through release of the highly adhesive cell-penetrating peptide and disruption of fluorescence resonance energy transfer. The H2O2 sensor selectively reacts with endogenous H2O2 in cell culture to monitor the oxidative burst of promyelocytes and in vivo to image lung inflammation. Targeting H2O2 has potential applications in imaging and therapy of diseases related to oxidative stress.
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