乙醇
脂肪变性
化学
绿原酸
酒
肝损伤
药理学
MAPK/ERK通路
生物化学
内科学
色谱法
医学
信号转导
作者
Xiaojun Li,Yueying Mu,Tingting Li,Yi-Yan Yang,Mei-Tuo Zhang,Yu-Sang Li,Wei Kevin Zhang,He-Bin Tang,Hongcai Shang
标识
DOI:10.1021/acs.jafc.5b03504
摘要
The present study aimed to evaluate the hepatoprotective effect and mechanism of action of Gynura procumbens on acute and chronic ethanol-induced liver injuries. Ethanol extract from G. procumbens stems (EEGS) attenuated acute ethanol-induced serum alanine aminotransferase levels and hepatic lipid accumulation. Therefore, EEGS was successively extracted by petroleum, ethyl acetate, and n-butyl alcohol. The results showed that the n-butyl alcohol extract was the active fraction of EEGS, and hence it was further fractionated on a polyamide glass column. The 60% ethanol-eluted fraction that contained 13.6% chlorogenic acid was the most active fraction, and its effect was further evaluated using a chronic model. Both the n-butyl alcohol extract and the 60% ethanol-eluted fraction inhibited chronic ethanol-induced hepatic lipid accumulation by modulating lipid metabolism-related regulators through MAPK/SREBP-1c-dependent and -independent signaling pathways and ameliorated liver steatosis. Our findings suggest that EEGS and one of its active ingredients, chlorogenic acid, may be developed as potential effective agents for ethanol-induced liver injury.
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